Journal: PLoS ONE

Article Title: MicroRNAs and histone deacetylase inhibition-mediated protection against inflammatory β-cell damage

doi: 10.1371/journal.pone.0203713

Figure Lengend Snippet: miRs regulated by cytokines and Givinostat.

Article Snippet: RNA quality and concentration was measured on a NanoDrop 1000 (Thermo Scientific). cDNA for qRT-PCR was prepared using the TaqMan ® Reverse Transcription kit (Applied Biosystems), according to the manufacturer’s protocol. cDNA for mature miR expression was prepared using TaqMan ® microRNA Reverse Transcription kit (Applied Biosystems) according to a protocol optimized for multiplex reactions with up to four miR primer sets.

Techniques:

Journal: eLife

Article Title: Calibration of cell-intrinsic interleukin-2 response thresholds guides design of a regulatory T cell biased agonist

doi: 10.7554/eLife.65777

Figure Lengend Snippet:

Article Snippet: For quantitative polymerase chain reaction (qPCR), Tregs and CD8+ T cells were stimulated for 4 hr with 200 nM cytokine prior to RNA isolation using RNeasy kit (QIAGEN). cDNA was generated using the iScript reverse transcriptase kit. qPCR was performed using PowerTrack SYBR Green (ThermoFisher) and analyzed on StepOnePlus Real-Time PCR System (Applied Biosystems).

Techniques: Expressing, Recombinant, Modification, Sequencing, Cell Isolation, Selection, Reverse Transcription, SYBR Green Assay, Flow Cytometry, Staining, Activation Assay, Software